Application

Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)

Optimal working dilutions must be determined by the end user.

This Anti-Connexin 43 Antibody, phospho-specific (Ser368) is validated for use in WB for the detection of Connexin 43.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: phospho-specific (Ser368)

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser368 of human Connexin 43.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 µg/mL BSA, with 50% glycerol.

Specificity

Recognizes Ser368 phosphorylated Connexin 43. Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap-junction communication is important in development and regulation of cell growth.

Storage and Stability

Maintain at -20°C for 12 months after date of receipt. Do not aliquot.